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KMID : 0617319980070010106
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Journal of Pharmacetical Sceiences Ewha Womans University
1998 Volume.7 No. 1 p.106 ~ p.111
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Differential Activation of T Cells by T-Cell Receptor Ligand Analogs
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Choi, Yunhi
Suh, Yujin/Kim, Kilhyoun
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Abstract
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Although CD4^+ T cell responses to protein-derived antigen have well been understood. the epitopes recognized by hapten-specific CD4^+ T cells have not been fully defined. In this study. we characterized the response of a T cell hybridoma (5D10.1B8) which is specific for a hapten. N-hydroxysuccinimidyl-4-azidobenzoate (HSAB) restricted by MHC class Ⅱ I-A^d. Using three different antigen presenting cells (APCs) expressing I-A^d. the role of class Ⅱ MHC proteins in haptenic antigen presentation and subsequent activation of 5D10.1B8 has been examined. Activation of 5D10.1B8 T cells by HSAB analogs was also performed. Our results show that each APC activated T cells differentially and that interleukin-2 (IL-2) augmented antigen-presenting ability of all the APCs, suggesting that increased expression of class Ⅱ MHC protein by IL-2 played an important role in HSAB presentation and T cell activation. Finally, early T cell receptor-dependent signals induced by HSAB or its analogs were examined by phosphotyrosine immunoblot analysis. and showed that tyrosine phosphorylation level of a 18-20 kD protein increased upon stimulation.
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